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Best are specific tutorials, and some people develop tutorials or host teaching demonstrations on video that walk users through various editing processes. For example, you can find tutorials on how to create a deep vignette and how to add vintage film to a photo.

My favorite tutorials cover the basics of creating an image. You can find tutorials on how to create the basic effects discussed in this chapter such as the following:

• Creating Depth with Vignette and Lens Flare

• Getting Beautiful Skin

• Adding Color and Lighting to a Photo

• Creating a Waterfall Background

• Adding Vintage Film to Photos

• Creating a Retro Vignette

• Making a Graduated Black-and-White Image

• Creating a Styled Look

• Using the Liquify Tools

• Adding Smoke and Dust

• Creating a Vintage Photo Look

• Making a Melted-Look Effect

• Using the Toy Camera Filter

• Blending Colors

• Creating a Retro Look and Feel

• Tweaking a Photo Using the Liquify Tools

• Adding Flicker and Glare

• Applying a Solid Texture

• Using Brush Strokes

• Creating a Silver Bokeh Effect

• Creating a Cube Using a Vector Brush

• Using Paint

• Creating a Dynamic Text Effect

• Using the Magic Wand Tool

• Making an Image Float

Because of the huge number of resources available on the Web today, new tutorials are added at an incredible pace.

Look for the Photoshop editing tutorial titles listed in Chapter 10 and the titles in this chapter to find the best resources, the ones that cover the features you need to master.

The best way to search for a tutorial is through the search box at the top of any topic pages. Type in the topic word or term, such as **watercolor** or **vector art**, and press Enter. This search will bring up all the tutorials for that term, as shown in Figure 6-10.

Figure 6-10: Search for the best Photoshop tutorials by using the search box at the top of the page.

## Creating Depth with Vignette and Lens Flare

Photoshop has a native Vignette command that enables you to apply a darkened border around the outside of the image. The Vignette effect can be used to create a classic cinematic look, such as the look of vintage movie footage.

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Click the image to enlarge.

What Can Photoshop Elements Do

Elements comes with a suite of editing tools that are designed to help amateur photographers, graphic designers, web designers, meme creators and Discord users work smarter and faster in Photoshop.

Clone Tool

With the Clone Tool you can easily and effectively duplicate an area of an image (like using a stamp). The Clone Tool works like magic and has many special features which you can learn more about later on.

The Clone Tool is listed in the Tools tab in the top left of the main interface. For more information on the tools and actions available in Photoshop Elements click here.

Color Picker

The Color Picker allows you to select any color in an image from an image, a color wheel, or any website.

To use the Color Picker just click anywhere in the image and select the color. If it is a picture of a specific color then just click on the image and select the color you want.

You can also copy and paste the image’s color to any application.

Filter Gallery

The Filter Gallery can be accessed from the Tools tab in the top left of the main interface and you can use it to find an image effect which you like.

To use the Filter Gallery select the Filter Gallery button in the top left corner and you will see a list of all the filters that are available.

Select Filter Options on the left side to see more details about the filter.

There are numerous filters that allow you to do the following:

Change the color of an image (Filter > Blur > Motion Blur),

Make an image colorful (Filter > Adjust Color > Brightness/Contrast),

Apply a cartoon style (Filter > Stylize > Cartoon),

Make an image look much brighter (Filter > Adjust > Brightness/Contrast),

Make the colors of an image look much brighter (Filter > Brightness/Contrast),

Apply a vintage style (Filter > Stylize > Vintage) and

Add a vintage look to an image (Filter > Adjust > Stylize > Retro).

The Filter Gallery also allows you to access special effects that are not available in the regular filters. These special effects include:

Nature (Filter > Stylize > Landscape),

Night (Filter > Stylize > Night),

Portraits (Filter > Stylize >
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In this project we will delineate peptide structures responsible for recognition of class II molecule by T lymphocytes and HLA-DR restricted helper cell response and class I molecules by cytolytic T cells. The results of our studies will provide new insights into regulation of the immune response by regulating of class I and class II peptides and type of T helper cells (Th) induced by these peptides. The studies will allow us to use these results to develop vaccines that are useful to induce optimal Th1 immune responses. In Aim 1, we will investigate the requirement of synergy and cross-reactivity between peptides of different molecules for induction of CTL in vitro. We will examine the Th response in the presence of an HLA- matched allopeptide with DRw52 in which an epitope from a self molecule is linked to an allopeptide derived from a foreign or tumor associated molecule. A separate study will examine the requirement of synergy and cross-reactivity between peptides of the same molecule for induction of Th by DRw52- presented allo-peptides. These studies are important in the design of vaccines for cancer, transplantation, and induction of autoimmune disease. In Aim 2, we will examine the role of the structure of peptides and the combined contribution of amino acids to CD4+ and CD8+ T cell help in inducing an optimal Th response in vitro and in vivo. We will determine whether peptide epitopes require the presence of polar residues to induce a Th response. Mutagenesis of peptides will be performed in each position of the class I and class II epitopes to determine the role of individual amino acids. In Aim 3, we will examine the role of the nature of the amino acid at the C-terminal positions of the peptide in the induction of a Th response by DRw52-presented peptides and the role of amino acids at the N-terminal of the peptide in recognition by class II molecules and activation of CTL. The experimental results will be correlated with in silico analyses to determine the structure of the binding region of the Th molecules to determine if particular class I or class II molecules can bind specific sites on the peptide molecules. We will also determine the structure of the epitopes of class I or class II molecules recognized by Th or CTL cells. The results will be correlated to structural information obtained from the in silico analyses. These studies will provide important information on the structure of the class I and class II molecules that

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Dear Editor,

We thank our esteemed colleagues for their critical and constructive comments on our manuscript \[[@CR1]\]. The recently described Japanese patient with AEF was referred to our hospital as a rapidly progressive bilateral exophthalmos, dysphasia and fever. Whereas a detailed description of the pathophysiological mechanisms was not presented in our original manuscript \[[@CR1]\], these issues have been addressed in our revised manuscript \[[@CR2]\]. To the best of our knowledge, this is the first report of an acute infectious encephalopathy associated with the administration of regional anesthesia and endotracheal intubation in the literature \[[@CR1]\]. The full text of this case report is shown in the Additional file [1](#MOESM1){ref-type=”media”}.

The results of microbiological and molecular examinations were negative. A novel case of AEF is always assumed to be bacterial with a rapidly progressive clinical course and fatal outcome. However, a definitive diagnosis was not possible due to the autopsy being refused by the family, and there was no medical documentation to confirm whether there was a pre-existing lung disease, as described in our previous report. Therefore, other infectious etiologies were excluded. Since we judged it highly unlikely that she died of an infectious disease, we concluded that the most likely cause of death was an acute infectious encephalopathy associated with endotracheal intubation and regional anesthesia. Cerebrospinal fluid (CSF) analysis revealed a lymphocytic pleocytosis, with protein levels elevated up to 28 mg/dL. Although we did not perform CSF polymerase chain reaction (PCR) analysis, we assumed that the lymphocytic pleocytosis was mainly attributed to aseptic meningitis. This was based on the clinical course of the previous case, and also because the lymphocytic pleocytosis was relatively mild. Importantly, CSF showed elevated [d]{.smallcaps}-dimer levels, confirming that our patient had coagulopathy. Although no autopsy was performed, the presence of subarachnoid hemorrhage, on initial imaging, showed increased possibility of an intracerebral infection, such as that of AEF. Although our team did not perform a brain autopsy, histological staining, it was assumed that the cause of death was a subarachnoid hemorrhage.

These findings were not an isolated

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